FERROUS IRON TEST REAGENT
May be irritating to eyes, skin and respiratory tract. Harmful if swallowed. Do not ingest or allow contact with skin or
eyes. Do not breathe dust. In case of contact, immediately flush eyes with water for 15 minutes. Remove contaminated
clothing. Wash skin with soap and plenty of water. In case of ingestion, give large quantities of water. In case of
inhalation, remove victim to fresh air. Call medic in all cases.
Add one powder pillow of ferrous iron test reagent(23) to one of the sample cells(7, fig. 2-5) containing the
water sample. This will be the treated sample. Swirl to mix.
Allow solution to stand for at least 2 minutes but not more than 10 minutes. If iron is present an orange
color will develop. Proceed to the next step while waiting.
Place the untreated cell of sample water (blank sample) into the cell holder well(5) in the
spectrophotometer. Put the "Iron (FerroVer® Method)" meter scale card(2) into the meter. Use the
wavelength dial(8) to adjust the wavelength to 510 nm. Adjust the LIGHT CONTROL knob(l0) until the
meter scale(14) reads 0.0 g/ml.
Remove the blank sample from the spectrophotometer. Place the treated sample in the cell holder well(5).
Read and record the mg/l of ferrous iron on the meter scale(14).
Interpretation of Results:
The recorded reading corresponds to the concentration of ferrous iron in the sample in mg/I.
The amount of ferric iron present can be determined as the difference between the amount of ferrous iron
determined in this and the results of a total iron test (para 184.108.40.206).
A large excess of iron will inhibit full color development. If the reading is at the high end of the scale, the
test should be done again with a diluted sample. Dilute the sample by starting with only 12.5 ml of sample
water and adding to it 12.5 ml of deionized water. Then proceed as above. Multiply the
spectrophotometer scale reading by two.
Refer to para 2.6.2 for shut down of the Spectrophotometer.
220.127.116.11. Total Iron Test.
Purpose of Test: To determine the total amount of iron present in water using the (1,1-Phenanthroline Method).
Range: 0.0 to 2 mg/l
Equipment Used: Found in Main Carrying Case, para 1.8, fig. 1-1
Spectrophotometer, (20), fig. 1-1
FerroVer® iron reagent for 25 ml sample, (22), fig. 1-1
Graduated cylinder, (31), fig. 1-1
General Techniques Used: Refer to paragraphs cited below as required by procedure.
Planning the Test, para 18.104.22.168
Cleaning Glassware, para 22.214.171.124
Handling and Storing Reagents, para 126.96.36.199
Collecting Water Samples, para 188.8.131.52
Mixing, para 184.108.40.206
Using Graduated Cylinders, para 220.127.116.11
Spectrophotometer, para 18.104.22.168