TM 10-6630-245-13&P
d.
General Techniques Used: Refer to paragraphs cited below as required by procedure.
Planning the Test, para 2.6.1.1
Cleaning Glassware, para 2.6.1.2
Handling and Storing Reagents, para 2.6.1.3
Collecting Water Samples, para 2.6.1.4
Mixing, para 2.6.1.5
Using Pipets, para 2.6.1.7
Titrating, para 2.6.1.8
e.
Test Procedure:
(1)
Rinse the sample bottle(l, fig. 2-10) at least 3 times with water from the source to be tested. Cap the
bottle(1).
(2)
Fill the sample bottle(1) with water to be tested. Do this by completely submerging the bottle(1) below the
surface of the water source with the cap still on. Remove the cap underwater. Let the bottle(1) fill. As the
bottle(1) is filling squeeze the sides and tap it to ensure no bubbles are trapped. Tightly put the cap back
on the bottle(1) while it is still underwater.
(3)
Remove the bottle(1) from the water and examine it closely for bubbles. If any are found repeat step (2).
NOTE
Steps (4), (5), and (6) below "fix" the oxygen in the sample so that the test will not be affected by
contact with oxygen in the air. Samples collected in the field can be fixed and carried to another
location for the remainder of the steps.
WARNING
MANGANESE SULFATE REAGENT
For test purposes only. May be harmful if swallowed. May irritate eyes, skin and mucous
membranes on contact. Do not ingest or allow contact with skin or eyes. Avoid breathing
vapors. In case of contact, flush eyes with water for at least 15 minutes. Get immediate medical
attention. Wash skin with soap and water. In case of ingestion, get immediate medical attention.
In case of inhalation, remove victim to fresh air.
(4)
Carefully remove the cap from the sample bottle(l) and add 8 drops of manganese sulfate solution(5).
Replace the cap on the sample bottle(l). If the manganese supplied in your kit comes with a dropper
bottle, drops can be measured directly from the bottle. Otherwise use the 1.0 ml dropping pipet(8) to
transfer the drops to the sample bottle(l). Rinse the dropping pipet(8) immediately afterwards.
WARNING
ALKALINE POTASSIUM IODIDE SOLUTION
Corrosive alkali. Harmful if swallowed or inhaled. Burns eyes, skin, nasal and respiratory
passages on contact. Vapor irritates eyes and respiratory passages. Do not ingest or allow
contact with skin or eyes. Avoid breathing vapors. In case of contact, flush eyes or skin with
plenty of water. For eyes, get medical attention. In case of ingestion, DO NOT induce vomiting.
Give large amounts of dilute [1: 4] vinegar, lemon, or grapefruit juice. Follow with milk or raw
egg whites beaten in water. Call medic immediately. In case of inhalation, remove victim to fresh
air.
(5)
Carefully remove the cap from the sample bottle(l) and add 8 drops of alkaline potassium iodide
solution(10). Replace the cap on the sample bottle(1). If the potassium iodide solution supplied in your kit
comes with a dropper bottle, drops can be measured directly from the bottle. Otherwise use the 1.0 ml
dropping pipet(8) to transfer the drops to the sample bottle(1). Rinse the dropping pipet(8) immediately
afterwards.
(6)
Mix by gently turning the sample bottle(l) upside down several times. Allow the precipitate to settle below
shoulder of the sample bottle(1).
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